生命科学研究部門 / Life science

2021

  • Miloš Havelka, Eitaro Sawayama, Taiju Saito, Kazutoshi Yoshitake, Daiki Saka, Toshinao Ineno, Shuichi Asakawa, Motohiro Takagi, Rie Goto, Takahiro Matsubara
    Chromosome-Scale Genome Assembly and Transcriptome Assembly of Kawakawa Euthynnus affinis; A Tuna-Like Species
    Front. Genet., 20 September 2021
    (2021).
  • Takao Hayashida, Kentaro Higuchi, Hiroshi Hashimoto, Yukinori Kazeto, Takeshi Eba, Sota Yoshikawa, Rie Goto, Kogen Okita, Toshinori Takashi, Keita Ishii, Takashi Yamashita, Kazunori Kumon, Koichiro Gena
    Gonadal sex differentiation and early ovarian/testicular development in cultured Pacific bluefin tuna, Thunnus orientalis (Temminck et Schlegel)
    Theriogenology Volume 173, 1 October 2021, Pages 56-63
    (2021).

2020

  • Hideo TAKAHARA, Rie GOTO, Taiju SAITO, Yasunori SAKURAI
    Mode of Infection with Larval Anisakid Nematodes in the Japanese Flying Squid, Todarodes Pacificus (Cephalopoda: Ommastrephidae)
    International Journal of Innovative Studies in Aquatic Biology and Fisheries Volume 6, Issue 3, 2020, PP 16-22
    (2020).
  • Ayumi Bandai, Takahiro Matsubara, Rie Goto, Taketo Hayakawa, Yukio Iwatsuki, Hiroyuki Motomura.
    Sexual dichromatism and dimorphism in the goatfish Parupeneus spilurus (Perciformes: Mullidae) in southern Japan
    Ichthyological Research 67, pp 203-211
    (2020).

2019

  • Taiju Saito, Rie Goto, Nicola Rivers, Etsuro Yamaha
    Production of Germ-Line Chimeras in Zebrafish
    Vertebrate Embryogenesis pp 327-341
    (2019).
  • Kentaro Higuchi, Yukinori Kazeto, Yuichi Ozaki, Toshiya Yamaguchi, Yukinori Shimada, Yoshiaki Ina, Satoshi Soma, Yoshitaka Sakakura, Rie Goto, Takahiro Matsubara, Issei Nishiki, Yuki Iwasaki, Motoshige Yasuike, Yoji Nakamura, Aiko Matsuura, Shukei Masuma, Tetsushi Sakuma, Takashi Yamamoto, Tetsuji Masaoka, Takanori Kobayashi, Atsushi Fujiwara, Koichiro Gen
    Targeted mutagenesisof the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)
    Scientific Reports volume 9, Article number:13871
    (2019).
  • Tapas Chakraborty, Sipra Mohapatra, Lin Yan Zhou, Kohei Ohta, Takahiro Matsubara, Taisen Iguchi, Yoshitaka Nagahama.
    Estrogen Receptor β2 Oversees Germ Cell Maintenance and Gonadal Sex Differentiation in Medaka, Oryzias latipes
    StemCellReports Volume 13, Issue 2, 13 August 2019, Pages 419-433
    (2019).
  • Kentaro Higuchi, Rie Goto, Junpei Konishi, Yoshiaki Ina, Yukinori Kazeto, Koichiro Gen.
    Early development of primordial germ cells in Pacific bluefin tuna Thunnus orientalis.
    Theriogenology Volume 131, June 2019, Pages 106-112
    (2019).

2018

  • Rie Goto,Taiju Saito,Takahiro Matsubara,Etsuro Yamaha.
    Microinjection of Marine Fish Eggs.
    Part of the Methods in Molecular Biology book series (MIMB, volume 1874)
    (2018).
  • M.A.N. Reza, Sipra Mohapatra, S. Shimizu, S. Kitamura, S. Harakawa, H. Kawakami, K. Nakayama, E. Sawayama, T. Matsubara, K. Ohta, T. Chakraborty.
    Molecular cloning, characterization and expression analysis of complement components in red sea bream (Pagrus major) after Edwardsiella tarda and red sea bream Iridovirus (RSIV) challenge.
    Fish and Shellfish Immunology. 82:286-295 [Corresponding author] (Impact Factor: 3.38).
    (2018).
  • Pocherniaieva K, Sidova M, Havelka M, Saito T, Psenicka M, Sindelka R, Kaspar V.
    Comparison of oocyte mRNA localization patterns in sterlet Acipenser ruthenus and African clawed frog Xenopus laevis.
    J Exp Zool B Mol Dev Evol. 2018 May;330(3):181-187. doi: 10.1002/jez.b.22802. Epub 2018 Apr 23.
    (2018).
  • Pocherniaieva Hilal Güralp, Taiju Saito, Martin Pšenička, Tomáš Tichopád, Karel Janko, Vojtěch Kašpar.
    The Timing and Characterization of Maternal to Zygote Transition and Mid-Blastula Transition in Sterlet Acipenser Ruthenus and A. ruthenus x Acipenser gueldenstaedtii Hybrid.
    Journal of Fisheries and Aquatic Sciences. DOI: 10.4194/1303-2712-v19_02_09
    (2018).
  • Diógenes Henrique de Siqueira-Silva, Taiju Saito, Amanda Pereira dos Santos-Silva, Raphael da Silva Costa, Martin Psenicka, George Shigueki Yasui.
    Biotechnology applied to fish reproduction: tools for conservation.
    Fish Physiol Biochem. 2018 Apr 29. doi: 10.1007/s10695-018-0506-0. [Epub ahead of print](MIMB, volume 1874)
    (2018).
  • Viktoriia Iegorova, Martin Psenicka, Ievgen Lebeda, Marek Rodina, Taiju Saito.
    Polyspermy produces viable haploid/diploid mosaics in sturgeon.
    Biology of Reploduction. doi: 10.1093/biolre/ioy092. [Epub ahead of print]
    (2018).
  • Saito, T., Guralp, H., Iegorova, V., Rodina, M., Psenicka, M.
    Elimination of primordial germ cells in sturgeon embryos by UV-irradiation.
    Biology of Reploduction. 2018 Apr 7. doi: 10.1093/biolre/ioy076. [Epub ahead of print]
    (2018).
  • Fatira, E., Havelka, M., Labbe, C., Depincé, A., Iegorova, V., Pšenička, M., Saito, T.
    Application of interspecific Somatic Cell Nuclear Transfer (iSCNT) in sturgeons and an unexpectedly produced gynogenetic sterlet with homozygous quadruple haploid.
    Scientific Reports. 2018 Apr 16;8(1):5997. doi: 10.1038/s41598-018-24376-1
    (2018).

2017

  • Piva LH, de Siqueira-Silva DH, Goes CAG, Fujimoto T, Saito T, Dragone LV, Senhorini JA, Porto-Foresti F, Ferraz JBS, Yasui GS.
    Triploid or hybrid tetra: Which is the ideal sterile host for surrogate technology?
    Theriogenology, 108:239-244. doi: 10.1016/j.theriogenology.2017.12.013.
    (2017).
  • Güralp, H., Pocherniaieva, K., Blecha, M., Policar, T., Pšenička, M., Saito, T.
    Development, and effect of water temperature on development rate, of pikeperch Sander lucioperca embryos.
    Theriogenology, Volume 104, December 2017, Pages 94-104
    (2017).
  • Mohapatra S., Chakraborty T., Reza M.A.N., Shimizu S., Matsubara T., Ohta K.
    Short-term starvation and realimentation helps stave off Edwardsiella tarda infection in red sea bream (Pagrus major).
    Comparative Biochemistry and Physiology: Volume 206, April 2017, Pages 42-53
    (2017).
  • Chakraborty T., Mohapatra S., Tobayama M., Ohta K., Ryu Y.W., Kazeto Y., Ohta K., Zhou L.Y., Nagahama Y., Matsubara T.
    Hatching enzymes disrupt aberrant gonadal degeneration by the autophagy/apoptosis cell fate decision.
    Scientific Reportsvolume 7, Article number: 3183.
    (2017).
  • Pandey, D. , Ryu, Y.W. , and Matsubara, T.
    Features of Sperm Motility and Circadian Rhythm in Japanese Anchovy (Engraulis japonicus).
    Fisheries and Aquaculture Journal8:203. doi: 10.4172/2150-3508.1000203.
    (2017).
  • Pandey, D. , Ryu, Y.W. , Goto, R., and Matsubara, T.
    Morphological and biochemical changes in oocytes during final oocyte maturation in Japanese anchovy (Engraulis japonicus).
    quacult.Sci, 65(1), 29-40.
    (2017).
  • Golpour, A., Broquard, C., Milla, C., Dadras, H., Baloch, A.R., Saito, T., Pšenička, M.
    Gonad histology and serum 11-KT profile during the annual reproductive cycle in sterlet sturgeon adult males, Acipenser ruthenus.
    Reproduction in Domestic Animals, Volume52, Issue2 April 2017, Pages 319-326
    (2017).
  • Fujimoto, T., Yamada, A., Kudo, Y., Nakaya, K., Okubo- Murata, M., Saito, T., Ninomiya, K., Inaba, M., Kuroda, M., Arai, K., Murakami, M.
    Development of nuclear DNA markers to characterize genetically diverse groups of Misgurnus anguillicaudatus and its closely related species.
    Fisheries Science September 2017, Volume 83, Issue 5, pp 743–756.
    (2017).

2016

  • Mohapatra S., Chakraborty T., Kacem R.H., Shimizu S., Matsubara T., Ohta K.
    Starvation: An Alternate Measure to Improve Immunity and Physiology of Red Sea Bream during Edwardsiella tarda Infection.
    Journal of Aquatic Research and Development S2:007. [# Authors contributed equally].
    (2016).
  • Zhao, Y., Fujimoto, T., Pšenička, M., Saito, T., Arai, K.
    Non-motile tetraploid spermatozoa of Misgurnus loach hybrids.
    Fisheries Science, 82(1), pp 127-135.
    (2016).
  • Robles, V., Riesco, MF., Psenicka, M., Saito, T., Valcarce, DG., Cabrita, E., Herráez, P.
    Biology of teleost primordial germ cells (PGCs) and spermatogonia: biotechnological applications.
    Volume 472, 1 April 2017, Pages 4-20
    (2016).
  • Güralp, H., Pocherniaieva, K., Blecha, M., Policar, T., Pšenička, M., Saito, T.
    Early embryonic development in pikeperch (Sander lucioperca) related to micromanipulation.
    Czech Journal of Animal Science 61(06):273-280
    (2016).
  • Güralp, H., Pocherniaieva, K., Blecha, M., Policar, T., Pšenička, M., Saito, T.
    The migration of primordial germ cells during late embryogenesis of pikeperch Sander lucioperca relative to blastomere transplantation.
    Czech Journal of Animal Science 62, 2017 (3): 121–129.
    (2016).

2015

  • Saito, T., Pšenička, M.
    Novel technique for visualizing primordial germ cells in sturgeons (Acipenser ruthenus, A. gueldenstaedtii, A. baerii, and Huso huso).
    Biology or Reproduction, 93(4), 1-7.
    (2015).
  • Linhartova, Z., Saito, T., and M. Psenicka.
    Sterilization of sterlet Acipenser ruthenus by using knockdown agent, antisense morpholino oligonucleotide, against dead end gene.
    Theriogenology, 84(7), 1246-1255.
    (2015).
  • Hou, J., Fujimoto, T., Saito, T., Yamaha, E., and Arai, K.
    Generation of clonal zebrafish line by androgenesis without egg irradiation.
    Scientific Reports, 5:13346. (IF 2015 = 5.578)
    (2015).
  • Goto R, Saito T, Kawakami Y, Kitauchi T, Takagi M, Todo T, Arai K, and Yamaha E.
    Visualization of primordial germ cells in the fertilized pelagic eggs of the barfin flounder Verasper moseri.
    International Journal of Developmental Biology, 59(10-12), 465-470.
    (2015).
  • Qui, Y., Sun, S., Charkraborty, T., Sun, L., Wei, J., Nagahama, Y., Wang, D. and Zhou, L.
    Fig1α favors ovarian differentiation by antagonizing spermatogenesis in a teleost, Nile tilapia (Oreochromis niloticus).
    PloS One 10: e0123900
    (2015).
  • Mohapatra, S., Chakraborty, T., Shimizu, S., Urasaki, S., Matsubara, T., Nagahama, Y. and Ohta, K.
    Starvation beneficially influences the liver physiology and nutrient metabolism in Edwardsiella tarda infected red sea bream (Pagrus major).
    Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol. 189: 1-10
    (2015).
  • Pšenička, M., Saito, T., Linhartová, Z. and Gazo, I.
    Isolation and transplantation of sturgeon early-stage germ cells.
    Theriogenology 83: 1085-1092
    (2015).
  • Tzung, K.W., Goto, R., Saju, J.M., Sreenivasan, R., Saito, T., Arai, K., Yamaha, E., Hossain, M.S., Calvert, M.E.K. and Orbán, L.
    Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation.
    Stem Cell Reports 4: 1-13
    (2015).
  • Hiramatsu, N., Todo, T., Sullivan, C.V., Schilling, J., Reading, B.J., Matsubara, T., Ryu, Y.W., Mizuta, H., Luo, W., Nishimiya, O., Wu, M., Mushirobira, Y., Yilmaz, O. and Hara, A.
    Ovarian yolk formation in fishes: Molecular mechanisms underlying formation of lipid droplets and vitellogenin-derived yolk proteins.
    General and Comparative Endocrinology Volume 221, 15 September 2015, Pages 9-15
    (2015).

2014

  • Linhartova, Z., Saito, T., and M. Psenicka.
    Embryogenesis, visualization and migration of primordial germ cells in tench (Tinca tinca).
    Journal of Applied Ichthyology 30.s1 (2014): 29-39. (IF 2012 = 0.902)
    (2014).
  • Zhao, Y., Saito, T., Pšenička, M., Fujimoto, T., and Arai, K.
    Comparison of spermatozoa parameters, fine structures, and energy-related factors among tetraploid, hyper-tetraploid, and hyper-triploid loaches (Misgurnus anguillicaudatus).
    Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 321(4), 198-206. (IF 2012 = 1.610)
    (2014).
  • Linhartova, Z., Rodina, M., Guralp, H., Gazo, I., Saito, T., and M. Psenicka.
    Isolation and cryopreservation of early stages of germ cells of tench (Tinca tinca).
    Czech Journal Of Animal Science, 59 (8): 381-390
    (2014).
  • Dzyuba, B., Boryshpolets, S., Cosson, J., Dzyuba, V., Fedorov, P., Saito, T., Pšenička, M., Linhart, O., and Rodina, M.
    Motility and fertilization ability of sterlet Acipenser ruthenus testicular sperm after cryopreservation.
    Cryobiology, 69(2), 339-341. (IF 2014 =1.643)
    (2014).
  • Hou, J., Saito, T., Fujimoto, T., Yamaha, E. and Arai, K.
    Androgenetic doubled haploids induced without irradiation of eggs in loach (Misgurnus anguillicaudatus).
    Aquaculture. 420-421(Supplement 1): S57-S63 (IF 2012 = 2.009)
    (2014).
  • Saito, T., Pšenička, M., Goto, R., Adachi, S., Inoue, K., Arai, K. and Yamaha, E.
    The origin and migration of primordial germ cells in sturgeons.
    PloS One 9: e86861
    (2014).

2013

  • Hiramatsu, N., Luo, W., Reading, B.J., Sullivan, C.V., Mizuta, H., Ryu, Y.-W., Nishimiya, O., Todo, T. and Hara, A.
    Multiple ovarian lipoprotein receptors in teleosts.
    Fish Physiol. Biochem. 39: 29-32
    (2013).

2012

  • Zhao, Y., Psenicka, M., Fujimoto, T., Saito, T., Yasui, GS., Yamaha, E., Arai, K.
    Motility, morphology, mitochondria and ATP content of diploid spermatozoa from sex-reversed clonal diploid and neo-tetraploid loach, Misgurnus anguillicaudatus.
    Journal of Applied Ichthyology. 28, 1006–1012. (IF 2011 = 0.869)
    (2012).
  • Kitauchi, T., Saito, T., Motomura, T., Arai, K., Yamaha, E.
    Distribution and function of germ plasm in cytoplasmic fragments from centrifuged eggs of the goldfish, Carassius auratus.
    Journal of Applied Ichthyology. 28, 998–1005. (IF 2011 = 0.869)
    (2012).
  • Kawakami, Y., Saito, T., Fujimoto, T., Goto-Kazeto, R., Takahashi, E., Adachi, S., Arai, K., Yamaha, E.
    Technical note: viability and motility of vitrified/thawed primordial germ cell isolated from common carp (Cyprinus carpio) somite embryos.
    Journal of Animal Science. 90, 495-500. (IF 2011 = 2.096)
    (2012).
  • Kawakami, Y., Ishihara, M., Saito, T., Fujimoto, T., Adachi, S., Arai, K., and Yamaha, E.
    Cryopreservation of green fluorescent protein (GFP)-labeled primordial germ cells with GFP fused to the 3′ untranslated region of the nanos gene by vitrification of Japanese eel (Anguilla japonica) somite stage embryos.
    Journal of Animal Science. (IF 2011 = 2.096)
    (2012).
  • Mizuno, K., Shimizu-Yamaguchi, S., Miura, C. and Miura, T.
    Method for efficiently obtaining fertilized eggs from the black scraper Thamnaconus modestus by natural spawning in captivity.
    Fisheries Science, September 2012, Volume 78, Issue 5, pp 1059–1064
    (2012).
  • Bhatta, S. , Iwai, T., Miura, C., Higuchi, M., Shimizu-Yamaguchi, S., Fukada, H. and Miura, T.
    Gonads directly regulate growth in teleosts.
    PNAS July 10, 2012 109 (28) 11408-11412
    (2012).
  • Celino, F. T., Shimizu-Yamaguchi, S. , Miura, C. and Miura, T.
    Proliferating spermatogonia are susceptible to reactive oxygen species attack in Japanese eel (Anguilla japonica).
    Biology of Reproduction, Volume 87, Issue 3, pp 1-9
    (2012).
  • Higuchi, M. , Celino, F. T., Shimizu-Yamaguchi, S., Miura, C. and Miura, T.
    Taurine plays an important role in the protection of spermatogonia from oxidative stress.
    Amino Acids, December 2012, Volume 43, Issue 6, pp 2359–2369
    (2012).
  • Goto, R., Saito, T., Takeda, T., Fujimoto, T., Takagi, M., Arai, K. and Yamaha, E.
    Germ cells are not the primary factor for sexual fate determination in goldfish.
    Developmental Biology 370, 98–109. (IF 2011 = 4.069)
    (2012).

2011

  • Saito, T., Goto-Kazeto, R., Kawakami, Y., Nomura, K., Tanaka, H., Adachi, S., Arai, K., and Yamaha, E.
    The mechanism for primordial germ-cell migration is conserved between Japanese eel and zebrafish.
    PLoS ONE 6, e24460–. (IF 2010 = 4.411)
    (2011).
  • Wong, T.T., Saito, T., Crodian, J., and Collodi, P.
    Zebrafish Germline Chimeras Produced by Transplantation of Ovarian Germ Cells into Sterile Host Larvae.
    Biology of Reproduction 84, 1190–1197. (IF 2010 = 3.870)
    (2011).
  • Kawakami, Y., Saito, T., Fujimoto, T., Goto-Kazeto, R., Takahashi, E., Adachi, S., Arai, K., and Yamaha, E.
    Visualization and motility of primordial germ cells using green fluorescent protein fused to 3’UTR of common carp nanos-related gene.
    Aquaculture 317, 245–250. (IF 2010 = 2.044)
    (2011).

2010

  • Fujimoto, T., Saito, T., Sakao, S., Arai, K., and Yamaha, E.
    Developmental potential of embryonic cells in a nucleocytoplasmic hybrid formed using a goldfish haploid nucleus and loach egg cytoplasm.
    Int. J. Dev. Biol. 54, 827–835. (IF 2010 = 2.856)
    (2010).
  • Goto-Kazeto, R., Saito, T., Takagi, M., Arai, K., and Yamaha, E.
    Isolation of teleost primordial germ cells using flow cytometry.
    Int. J. Dev. Biol. 54, 1485–1490. (IF 2010 = 2.856)
    (2010).
  • Kawakami, Y., Goto-Kazeto, R., Saito, T., Fujimoto, T., Higaki, S., Takahashi, Y., Arai, K., and Yamaha, E.
    Generation of germ-line chimera zebrafish using primordial germ cells isolated from cultured blastomeres and cryopreserved embryoids.
    Int. J. Dev. Biol. 54, 1493–1501. (IF 2010 = 2.856)
    (2010).
  • Saito, T., Goto-Kazeto, R., Fujimoto, T., Kawakami, Y., Arai, K., and Yamaha, E.
    Inter-species transplantation and migration of primordial germ cells in cyprinid fish.
    Int. J. Dev. Biol. 54, 1479–1484. (IF 2010 = 2.856)
    (2010).
  • Yamaha, E., Goto-Kazeto, R., Saito, T., Kawakami, Y., Fujimoto, T., Adachi, S., and Arai, K.
    Primordial germ cell in teleost fish with special references to its specification and migration.
    Journal of Applied Ichthyology 26, 816–822. (IF 2010 = 0.945)
    (2010).

2009

  • Yoshikawa, H., Morishima, K., Fujimoto, T., Saito, T., Kobayashi, T., Yamaha, E., and Arai, K.
    Chromosome Doubling in Early Spermatogonia Produces Diploid Spermatozoa in a Natural Clonal Fish.
    Biology of Reproduction 80, 973–979. (IF 2009 = 3.300)
    (2009).

2008

  • Saito, T., Goto-Kazeto, R., Arai, K., and Yamaha, E.
    Xenogenesis in teleost fish through generation of germ-line chimeras by single primordial germ cell transplantation.
    Biology of Reproduction 78, 159–166. (IF 2008 = 3.469)
    (2008).